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Anti-CD3 epsilon/CD3e Polyclonal Antibody 2
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产品编号 TMAB-00352
Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 CD3 epsilon/CD3e。Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-CD3 epsilon/CD3e Polyclonal Antibody 2
Anti-CD3 epsilon/CD3e Polyclonal Antibody 2
还可以产品编号 TMAB-00352
Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 CD3 epsilon/CD3e。Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat,MACFA) |
| 验证活性 | 1. Tissue/cell: mouse lymphoma tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CD3 Polyclonal Antibody, Unconjugated (TMAB-00352) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C. 2. Sample: Lane 1: Recombinant mouse CD3E protein, C-mFc (HEK293) Primary: Anti-CD3E (TMAB-00352) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 36 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. |
| 免疫原 | KLH conjugated synthetic peptide: mouse CD3E |
| 抗原种属 | Mouse |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Regulatory T Cells,CD,cd3,Human Lineage Negative |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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