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Anti-CD166/ALCAM Antibody (7F950)
还可以产品编号 TMAY-03777
Anti-CD166/ALCAM Antibody (7F950) 是一种 Rabbit 抗体，靶向 CD166/ALCAM。Anti-CD166/ALCAM Antibody (7F950) 可用于 WB,ELISA,IP。
Anti-CD166/ALCAM Antibody (7F950)
Anti-CD166/ALCAM Antibody (7F950)
还可以产品编号 TMAY-03777
Anti-CD166/ALCAM Antibody (7F950) 是一种 Rabbit 抗体，靶向 CD166/ALCAM。Anti-CD166/ALCAM Antibody (7F950) 可用于 WB,ELISA,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CD166/ALCAM Antibody (7F950) is a Rabbit antibody targeting CD166/ALCAM. Anti-CD166/ALCAM Antibody (7F950) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 7F950 |
| 交叉反应 | Rat |
| 验证活性 | 1. Anti-ALCAM rabbit monoclonal antibody at 1:500 dilution. -Lane A: SH-SY5Y Whole Cell Lysate. -Lane B: Daudi Whole Cell Lysate. -Lane C: HuT78 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:65 kDa. -Observed band size:100 kDa. 2. rat，hALCAM was immunoprecipitated using: -Lane A:0.5 mg SH-SY5Y Whole Cell Lysate. -2 µL anti-rat，hALCAM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-rat，hALCAM rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 65 kDa. -Observed band size: 100 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Rat CD166 / ALCAM (TMPY-02936; O35112; Met1-Lys527). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Rat CD166 / ALCAM protein (TMPY-02936) |
| 抗原种属 | Rat |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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