Anti-CD163 Antibody (5V810) is a Rabbit antibody targeting CD163. Anti-CD163 Antibody (5V810) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

5V810

Human,Mouse,Rat

1. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Rat Lung tissue lysates
Primary: Anti-CD163 (TMAB-00339) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 170 kDa
2. 25 μg total protein per Lane of various lysates probed with CD163 monoclonal antibody, unconjugated (TMAB-00339) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Monoclonal Antibody, Unconjugated (TMAB-00339) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human CD163

Human

Innate and adaptive immunity,Myeloid Cells,Macrophage / Inflamm.