购物车
- 全部删除
您的购物车当前为空
Anti-CD146/MCAM Antibody (8V819)
还可以产品编号 TMAY-00295
Anti-CD146/MCAM Antibody (8V819) 是一种 Rabbit 抗体，靶向 CD146/MCAM。Anti-CD146/MCAM Antibody (8V819) 可用于 ELISA,ELISA(Det),IHC-P,FCM,ICC/IF。
Anti-CD146/MCAM Antibody (8V819)
Anti-CD146/MCAM Antibody (8V819)
还可以产品编号 TMAY-00295
Anti-CD146/MCAM Antibody (8V819) 是一种 Rabbit 抗体，靶向 CD146/MCAM。Anti-CD146/MCAM Antibody (8V819) 可用于 ELISA,ELISA(Det),IHC-P,FCM,ICC/IF。
产品信息正在更新中，如果您想购买，请点击大包装&定制按钮.
大包装 & 定制
联系我们获取更多批次信息
资源下载
产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CD146/MCAM Antibody (8V819) is a Rabbit antibody targeting CD146/MCAM. Anti-CD146/MCAM Antibody (8V819) can be used in ELISA,ELISA(Det),IHC-P,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8V819 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200). 2. Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of endothelial cells (red arrow) (1:200). 3. Immunofluorescence staining of Human CD146 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD146 monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. 4. Flow cytometric analysis of anti-CD146 on HeLa cells. HeLa cells were detached using 1X trypsin, washed, then stained with purified rabbit anti-CD146. Second step staining with goat anti-rabbit IgG FC polyclonal antibody. |
| 应用 | ELISA,ELISA(Det),IHC-P,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; TMPY-01034; NP_006491.2; Met 1-Gly 559) . |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/HRP/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Human CD146 protein (TMPY-01034) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
评论列表
预览
Related Tags: buy Anti-CD146/MCAM Antibody (8V819) | purchase Anti-CD146/MCAM Antibody (8V819) | Anti-CD146/MCAM Antibody (8V819) cost | order Anti-CD146/MCAM Antibody (8V819)
嗨！有任何问题？点我咨询
评论内容