Anti-Caspase-1 p10 Polyclonal Antibody 2 is a Rabbit antibody targeting Caspase-1 p10. Anti-Caspase-1 p10 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Rat (predicted:Mouse)

1. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-Caspase-1 P10 antibody (TMAB-00281)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Lane 1: Small intestine (Rat) Lysate at 40 μg
Lane 2: Urinary bladder (Rat) Lysate at 40 μg
Primary: Anti-Caspase-1 P10 (TMAB-00281) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/10 kDa
Observed band size: 45/10 kDa

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: mouse Caspase-1 P10

Mouse

Response to hypoxia,Caspases,Metabolism,Caspases,Caspases,Apoptosis,Hypoxia