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Anti-Carbonic Anhydrase 8 Antibody (6D426)
还可以产品编号 TMAY-00873
Anti-Carbonic Anhydrase 8 Antibody (6D426) 是一种 Rabbit 抗体，靶向 Carbonic Anhydrase 8。Anti-Carbonic Anhydrase 8 Antibody (6D426) 可用于 WB,IP。
Anti-Carbonic Anhydrase 8 Antibody (6D426)
Anti-Carbonic Anhydrase 8 Antibody (6D426)
还可以产品编号 TMAY-00873
Anti-Carbonic Anhydrase 8 Antibody (6D426) 是一种 Rabbit 抗体，靶向 Carbonic Anhydrase 8。Anti-Carbonic Anhydrase 8 Antibody (6D426) 可用于 WB,IP。
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产品介绍
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抗原信息
| 产品描述 | Anti-Carbonic Anhydrase 8 Antibody (6D426) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (6D426) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6D426 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-CA8 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:38 kDa. 2. CA8 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg A549 Whole Cell Lysate -0.5 µL anti-CA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CA8 rabbit monoclonal antibody, at 1:150 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 37 kDa. -Observed band size: 37 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; TMPY-01706; NP_004047.3; Met1-Gln290). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Carbonic Anhydrase VIII / CA8 protein (TMPY-01706) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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