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Anti-Carbonic Anhydrase 8 Antibody (2N156)
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产品编号 TMAY-03451
别名 wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993
Anti-Carbonic Anhydrase 8 Antibody (2N156) 是一种 Rabbit 抗体，靶向 Carbonic Anhydrase 8。Anti-Carbonic Anhydrase 8 Antibody (2N156) 可用于 WB,IP。
Anti-Carbonic Anhydrase 8 Antibody (2N156)
331 Anti-Carbonic Anhydrase 8 Antibody (2N156)
还可以产品编号 TMAY-03451 别名 wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993
Anti-Carbonic Anhydrase 8 Antibody (2N156) 是一种 Rabbit 抗体，靶向 Carbonic Anhydrase 8。Anti-Carbonic Anhydrase 8 Antibody (2N156) 可用于 WB,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 2,284 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Carbonic Anhydrase 8 Antibody (2N156) is a Rabbit antibody targeting Carbonic Anhydrase 8. Anti-Carbonic Anhydrase 8 Antibody (2N156) can be used in WB,IP. |
| 别名 | wdl, RP23-180H12.1, Carp, carbonic anhydrase VIII, Car8, Cals1, Cals, Ca8, AW546993 |
| Ig Type | Rabbit IgG |
| 克隆号 | 2N156 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Anti-CA8 rabbit monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell Lysate. -Lane B: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:36 kDa. 2. Mouse CA8 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -Lane B:0.5 mg A549 Whole Cell Lysate. -2 µL anti-Mouse CA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse CA8 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size: 37 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carbonic Anhydrase VIII / CA8 (rM Carbonic Anhydrase VIII / CA8; TMPY-02301; P28651; Met1-Gln291). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Mouse Carbonic Anhydrase VIII / CA8 protein (TMPY-02301) |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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