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Anti-Carbonic Anhydrase 2 Antibody (8K348)
还可以产品编号 TMAY-00879
Anti-Carbonic Anhydrase 2 Antibody (8K348) 是一种 Rabbit 抗体，靶向 Carbonic Anhydrase 2。Anti-Carbonic Anhydrase 2 Antibody (8K348) 可用于 WB,IP。
Anti-Carbonic Anhydrase 2 Antibody (8K348)
Anti-Carbonic Anhydrase 2 Antibody (8K348)
还可以产品编号 TMAY-00879
Anti-Carbonic Anhydrase 2 Antibody (8K348) 是一种 Rabbit 抗体，靶向 Carbonic Anhydrase 2。Anti-Carbonic Anhydrase 2 Antibody (8K348) 可用于 WB,IP。
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| 产品描述 | Anti-Carbonic Anhydrase 2 Antibody (8K348) is a Rabbit antibody targeting Carbonic Anhydrase 2. Anti-Carbonic Anhydrase 2 Antibody (8K348) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8K348 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-CA2 rabbit monoclonal antibody at 1:10000 dilution. -Lane A: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:29 kDa. -Observed band size:30 kDa. 2. CA2 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -2 µL anti-CA2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CA2 rabbit monoclonal antibody, at 1:2000 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 29 kDa. -Observed band size: 29 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:10000-1:50000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase II / CA2 (rh Carbonic Anhydrase II / CA2; TMPY-01735; NP_000058.1; Met1-Lys260). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Carbonic Anhydrase II / CA2 protein (TMPY-01735) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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