Anti-C-Kit Antibody (7A806) is a Rabbit antibody targeting C-Kit. Anti-C-Kit Antibody (7A806) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

7A806

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Kit Monoclonal Antibody, Unconjugated (TMAB-00436) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Kit Monoclonal Antibody, Unconjugated (TMAB-00436) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Kit Monoclonal Antibody, Unconjugated (TMAB-00436) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Kit Monoclonal Antibody, Unconjugated (TMAB-00436) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Kit Monoclonal Antibody, Unconjugated (TMAB-00436) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Kit Monoclonal Antibody, Unconjugated (TMAB-00436) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (c-Kit) monoclonal Antibody, unconjugated (TMAB-00436) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
8. 25 μg total protein per Lane of various lysates probed with c-Kit monoclonal antibody, unconjugated (TMAB-00436) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: human c-Kit protein, around C-terminal 200aa

Human

KIT

Mast/stem cell growth factor receptor Kit

Growth factor receptors,Receptor tyrosine kinases,Hematopoietic system development,Germ cell markers,Cytokines,Tyrosine Kinase Receptors,Receptor Tyrosine Kinases,Embryonic Germ Cells,HSC markers,Mast Cell Lineage,Surface Molecules,Tumor Associated

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate.