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顶部生物活性储存和溶解信息计算器

Anti-BrdU Antibody (8W188)

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Anti-BrdU Antibody (8W188)
产品编号 TMAC-00474
别名 Budr, Bromodeoxyuridine

Anti-BrdU Antibody (8W188) 是一种 Mouse 抗体，靶向 BrdU。Anti-BrdU Antibody (8W188) 可用于 IF,IHC-P,FCM。

Anti-BrdU Antibody (8W188)

Anti-BrdU Antibody (8W188)

Rating icon 还可以
Anti-BrdU Antibody (8W188)
产品编号 TMAC-00474 别名 Budr, Bromodeoxyuridine

Anti-BrdU Antibody (8W188) 是一种 Mouse 抗体，靶向 BrdU。Anti-BrdU Antibody (8W188) 可用于 IF,IHC-P,FCM。

规格价格库存数量
50 μL
¥ 1,985
5日内发货
100 μL
¥ 2,980
5日内发货
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产品介绍

生物活性
产品描述
Anti-BrdU Antibody (8W188) is a Mouse antibody targeting BrdU. Anti-BrdU Antibody (8W188) can be used in IF,IHC-P,FCM.
别名Budr, Bromodeoxyuridine
交叉反应
Bromodeoxyuridine
验证活性
1. Immunofluorescence staining of methanol-fixed BrdU-labeled HeLa cells showing nuclear localization.
2. Intracellular FCM analysis of fixed and permeabilized untreated (dotted blue histogram) and BrdU treated (solid orange histogram) K-562 cells. Green line histogram represents the isotype control, normal mouse IgG1.
应用
IF
IHC-P
FCM
推荐剂量
IHC-P: 1:50-500; FCM: 1 μg per 1 x 106 cells
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdUrd immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Peptide
存储&运输
储存方式Store at 2°C-8°C for 12 months, do not freeze.
运输方式Shipping with blue ice.

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