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Anti-BCL-XL Antibody (3V974)
还可以产品编号 TMAY-00850
Anti-BCL-XL Antibody (3V974) 是一种 Mouse 抗体，靶向 BCL-XL。Anti-BCL-XL Antibody (3V974) 可用于 ELISA。
Anti-BCL-XL Antibody (3V974)
Anti-BCL-XL Antibody (3V974)
还可以产品编号 TMAY-00850
Anti-BCL-XL Antibody (3V974) 是一种 Mouse 抗体，靶向 BCL-XL。Anti-BCL-XL Antibody (3V974) 可用于 ELISA。
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偶联与修饰
抗原信息
| 产品描述 | Anti-BCL-XL Antibody (3V974) is a Mouse antibody targeting BCL-XL. Anti-BCL-XL Antibody (3V974) can be used in ELISA. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 3V974 |
| 交叉反应 | Human |
| 验证活性 | Flow cytometric analysis of Purified anti-Human BCL2L1 (Bcl-xL) on HeLa cells. HeLa cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-BCL2L1 (Bcl-xL) (Bold line hisgram), then stained with a FITC-conjugated second step antibody. To demonstrate specificity of staining the binding of Anti-BCL-XL Antibody was blocked by the preincubation of the purified antibody with molar excess of recombinant human BCL2L1 (Bcl-xL) (5 µg) for 1 hour (dashed line hisgram). |
| 应用 | ELISA |
| 推荐剂量 | ELISA: 1:1000-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Bcl-XL / BCL2L1 (rh Bcl-XL / BCL2L1; TMPY-02431; NP_612815.1; Met 1-Arg 212). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is characterized as an important factor in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Bcl-XL / BCL2L1 protein (TMPY-02431) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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