Anti-BAX Antibody (5X827)

Name: Anti-BAX Antibody (5X827)
Brand: TargetMol
SKU: TMAB-00190
Rating: 4.5 (1 reviews)

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产品编号 TMAB-00190

Anti-BAX Antibody (5X827) 是一种 Rabbit 抗体，靶向 BAX。Anti-BAX Antibody (5X827) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-BAX Antibody (5X827)

还可以

产品编号 TMAB-00190

Anti-BAX Antibody (5X827) 是一种 Rabbit 抗体，靶向 BAX。Anti-BAX Antibody (5X827) 可用于 WB,IHC-P,IHC-Fr,IF。

规格	价格	库存
25 μL	¥ 785	5日内发货
50 μL	¥ 1,390	5日内发货
100 μL	¥ 2,490	5日内发货

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产品描述	Anti-BAX Antibody (5X827) is a Rabbit antibody targeting BAX. Anti-BAX Antibody (5X827) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type	IgG
克隆号	5X827
交叉反应	Rat,Mouse,Human
验证活性	1. 25 μg total protein per Lane of various lysates probed with Bax monoclonal antibody, unconjugated (TMAB-00190) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 15. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 16. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 17. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 18. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 19. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (TMAB-00190) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
应用	WB IHC-P IHC-Fr IF
推荐剂量	WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
抗体种类	Monoclonal
宿主来源	Rabbit
亚细胞定位	Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Isoform Beta: Cytoplasm. Isoform Gamma: Cytoplasm. Isoform Delta: Cytoplasm (Potential).
构建方式	Recombinant Antibody
纯化方式	Protein A purified
性状	Liquid
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景	The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].

抗原信息

免疫原	A synthesized peptide: human BAX
抗原种属	Human
基因ID	581
Uniprot ID	Q07812
研究领域	Bcl 2 family,Metabolism,Bcl 2 family,Associated Proteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis