Anti-B7-H3 Antibody (8G959) is an antibody targeting B7-H3. Anti-B7-H3 Antibody (8G959) can be used in ELISA.

hIgG1

8G959

Human, Cynomolgus

1. The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276 at 2 μg/mL can bind Anti-CD276 recombinant antibody. The EC50 is 4.299-5.373 ng/mL.
2. The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276 at 2 μg/mL can bind Anti-CD276 recombinant antibody. The EC50 is 47.12-54.16 ng/mL.
3. Overlay Peak curve showing MCF7 cells stained with TMAH-00187MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA:1:5000-1:50000.

Monoclonal

Unconjugated

Recombinant Protein: Human CD276 Protein

Human

CD276

Cancer, Immunology, Stem cells