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Anti-ATP5A1 Antibody (2P206)
还可以产品编号 TMAH-00092
Anti-ATP5A1 Antibody (2P206) 是一种抗体，靶向 ATP5A1。Anti-ATP5A1 Antibody (2P206) 可用于 ELISA, WB, IHC。
Anti-ATP5A1 Antibody (2P206)
Anti-ATP5A1 Antibody (2P206)
还可以产品编号 TMAH-00092
Anti-ATP5A1 Antibody (2P206) 是一种抗体，靶向 ATP5A1。Anti-ATP5A1 Antibody (2P206) 可用于 ELISA, WB, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ATP5A1 Antibody (2P206) is an antibody targeting ATP5A1. Anti-ATP5A1 Antibody (2P206) can be used in ELISA, WB, IHC. |
| Ig Type | Rabbit IgG |
| 克隆号 | 2P206 |
| 交叉反应 | Human, Mouse |
| 验证活性 | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, Mouse Heart tissue, Mouse Brain tissue -All lanes: ATP5F1A antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 60, 55, 58 kDa -Observed band size: 60 kDa 2. IHC image of TMAH-00092 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00092 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, WB, IHC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Mitochondrion. Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Cell membrane; Peripheral membrane protein; Extracellular side. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human ATP5A1 |
| 抗原种属 | Human |
| 基因ID | 498 |
| Uniprot ID | |
| 研究领域 | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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