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顶部生物活性储存和溶解信息计算器

Anti-Apaf-1 Antibody (9E241)

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产品编号 TMAC-00235

别名 KIAA0413, CED4, CED 4, Apoptotic protease-activating factor 1, Apoptotic protease activating factor, Apoptotic peptidase activating factor 1, APAF1, APAF 1, APAF

Anti-Apaf-1 Antibody (9E241) 是一种 Rabbit 抗体，靶向 Apaf-1。Anti-Apaf-1 Antibody (9E241) 可用于 ICC/IF,IHC,WB。

Anti-Apaf-1 Antibody (9E241)

Anti-Apaf-1 Antibody (9E241)

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Rating icon 还可以
产品编号 TMAC-00235 别名 KIAA0413, CED4, CED 4, Apoptotic protease-activating factor 1, Apoptotic protease activating factor, Apoptotic peptidase activating factor 1, APAF1, APAF 1, APAF

Anti-Apaf-1 Antibody (9E241) 是一种 Rabbit 抗体，靶向 Apaf-1。Anti-Apaf-1 Antibody (9E241) 可用于 ICC/IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-Apaf-1 Antibody (9E241) is a Rabbit antibody targeting Apaf-1. Anti-Apaf-1 Antibody (9E241) can be used in ICC/IF,IHC,WB.
别名KIAA0413, CED4, CED 4, Apoptotic protease-activating factor 1, Apoptotic protease activating factor, Apoptotic peptidase activating factor 1, APAF1, APAF 1, APAF
Ig Type
IgG
克隆号
9E241
交叉反应
Human,Mouse
验证活性
1. Western blot analysis of Apaf-1 on HUVEC cell lysates using anti-Apaf-1 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Apaf-1 antibody. Counter stained with hematoxylin.
6. ICC staining Apaf-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Apaf-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Apaf-1 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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应用ICC/IFIHCWB
推荐剂量
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg2+-binding site). Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFkB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKg. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFkB in response to bacterial lipopoly-saccharides.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
O14727
化学信息
分子量Theoretical: 141 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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