Anti-Annexin A10 Polyclonal Antibody is a Rabbit antibody targeting Annexin A10. Anti-Annexin A10 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Cow,Horse)

1. Blank control: A549.
Primary Antibody (green line): Rabbit Anti-Annexin A10 antibody (TMAB-02584)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 5,000 events was performed.
2. Sample:
A549 (Human) Cell Lysate at 30 μg
Hela (Human) Cell Lysate at 30 μg
U87MG (Human) Cell Lysate at 30 μg
Primary: Anti-Annexin A10 (TMAB-02584) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Annexin A10

Human

ANXA10

Annexin A10