Anti-Acetyl-Histone H3.1 (Lys14) Antibody (1C240) is an antibody targeting Acetyl-Histone H3.1 (Lys14). Anti-Acetyl-Histone H3.1 (Lys14) Antibody (1C240) can be used in ELISA, WB, ICC, IF.

Rabbit IgG

1C240

Human, Rat

1. Western Blot
-Positive WB detected in Hela whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue
-All lanes Acetyl-Histone H3.1(K14)antibody at 0.75μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 15 KDa
-Observed band size: 15 KDa
2. Immunocytochemistry analysis of TMAH-00010 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with TMAH-00010 at 1:46,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

WB:1:500-1:2000; ICC:1:50-1:500; IF:1:30-1:200.

Monoclonal

Unconjugated

A synthetic peptide

Human

HIST1H3A

Epigenetics and Nuclear Signaling