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顶部生物活性储存和溶解信息计算器

Anti-ACBD6 Antibody (7G53)

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Anti-ACBD6 Antibody (7G53)
产品编号 TMAY-01651
别名 acyl-CoA binding domain containing 6

Anti-ACBD6 Antibody (7G53) 是一种 Mouse 抗体，靶向 ACBD6。Anti-ACBD6 Antibody (7G53) 可用于 WB,ELISA,ELISA(Det),IP。

Anti-ACBD6 Antibody (7G53)
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Anti-ACBD6 Antibody (7G53)

Rating icon 还可以
Anti-ACBD6 Antibody (7G53)
产品编号 TMAY-01651 别名 acyl-CoA binding domain containing 6

Anti-ACBD6 Antibody (7G53) 是一种 Mouse 抗体，靶向 ACBD6。Anti-ACBD6 Antibody (7G53) 可用于 WB,ELISA,ELISA(Det),IP。

规格价格库存数量
20 μL
¥ 585
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100 μL
¥ 1,694
5日内发货
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产品介绍

生物活性
产品描述
Anti-ACBD6 Antibody (7G53) is a Mouse antibody targeting ACBD6. Anti-ACBD6 Antibody (7G53) can be used in WB,ELISA,ELISA(Det),IP.
别名acyl-CoA binding domain containing 6
Ig Type
Mouse IgG1
克隆号
7G53
交叉反应
Human
验证活性
1. ACBD6 was immunoprecipitated using:
-Lane A:0.5 mg hela Whole Cell Lysate
-0.5 µL anti-ACBD6 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-ACBD6 mouse monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 31 kDa.
-Observed band size: 31 kDa.
2. Anti-ACBD6 mouse monoclonal antibody at 1:500 dilution.
-Lane A: 293T Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:31 kDa.
-Observed band size:35 kDa
应用
WB,ELISA,ELISA(Det),IP
推荐剂量
WB: 1:500-1:1000; ELISA: 1:5000-1:10000; IP: 1-2 μL/mg of lysate; ELISA(Det): 1:5000-1:50000
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACBD6 (rh ACBD6; TMPY-01345; NP_115736.1; Met 1-Ala 282). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Human acyl-coenzyme A binding domain-containing member 6 (ACBD6) is a modular protein that carries an acyl-CoA binding domain at its N terminus and two ankyrin motifs at its C terminus. In mammals, there are six members of the acyl-CoA binding domain-containing (ACBD) family, and their annotation is not uniform. All six ACBD proteins contain an ACB domain at the N terminus, but they do not share significant homology at the C-terminal region. ACBD6 is a 32 kDa protein that is predicted by sequence analysis to carry an ACB domain between residues 42 and 125 and two ANK motifs at its C terminus. This protein binds long-chain acyl-CoAs with a strong preference for unsaturated, C18:1-CoA and C20:4-CoA, over saturated, C16:0-CoA, acyl species. ACBD6 is not a ubiquitous protein, but it is expressed in hematopoietic tissues and appears to be restricted to primitive stem cells present in those tissues with functions in blood and vessel development. ACBD6 was detected in bone marrow, spleen, placenta, cord blood, circulating CD34+ progenitors, and embryonic-like stem cells derived from placenta. In placenta, the protein was only detected in CD34+ progenitor cells present in blood and CD31+ endothelial cells surrounding the blood vessels. These cells were also positive for the marker CD133, and they probably constitute hemangiogenic stem cells, precursors of both blood and vessels. We propose that human ACBD6 represents a cellular marker for primitive progenitor cells with functions in hematopoiesis and vascular endothelium development.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human ACBD6 protein (TMPY-01345)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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