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Anti-14-3-3 epsilon Antibody (2S60)
还可以产品编号 TMAY-01249
Anti-14-3-3 epsilon Antibody (2S60) 是一种 Mouse 抗体，靶向 14-3-3 epsilon。Anti-14-3-3 epsilon Antibody (2S60) 可用于 WB,IP。
Anti-14-3-3 epsilon Antibody (2S60)
Anti-14-3-3 epsilon Antibody (2S60)
还可以产品编号 TMAY-01249
Anti-14-3-3 epsilon Antibody (2S60) 是一种 Mouse 抗体，靶向 14-3-3 epsilon。Anti-14-3-3 epsilon Antibody (2S60) 可用于 WB,IP。
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| 产品描述 | Anti-14-3-3 epsilon Antibody (2S60) is a Mouse antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Antibody (2S60) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 2S60 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-YWHAE mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: U-251 MG Whole Cell Lysate. -Lane D: A375 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:29 kDa. -Observed band size:33 kDa. 2. YWHAE was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate. -Lane C:0.5 mg SH-SY5Y Whole Cell Lysate. -4 µL anti-YWHAE mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-YWHAE mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 34 kDa. -Observed band size:34 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: 1-5 µL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 epsilon (rh 14-3-3 epsilon; TMPY-01274; NP_006752.1; Met1-Gln255). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human 14-3-3 epsilon Protein (TMPY-01274) |
| 抗原种属 | Human |
| 研究领域 | phospho-Serine/phospho-Threonine Binding Proteins, Adaptor Proteins, Apoptosis Adaptor Proteins |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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