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Anti-14-3-3 beta/alpha Antibody (7X437)
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产品编号 TMAC-00005
别名 YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β
Anti-14-3-3 beta/alpha Antibody (7X437) 是一种 Rabbit 抗体，靶向 14-3-3 beta/alpha。Anti-14-3-3 beta/alpha Antibody (7X437) 可用于 WB,IHC。
Anti-14-3-3 beta/alpha Antibody (7X437)
Anti-14-3-3 beta/alpha Antibody (7X437)
还可以产品编号 TMAC-00005 别名 YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β
Anti-14-3-3 beta/alpha Antibody (7X437) 是一种 Rabbit 抗体，靶向 14-3-3 beta/alpha。Anti-14-3-3 beta/alpha Antibody (7X437) 可用于 WB,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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| 产品描述 | Anti-14-3-3 beta/alpha Antibody (7X437) is a Rabbit antibody targeting 14-3-3 beta/alpha. Anti-14-3-3 beta/alpha Antibody (7X437) can be used in WB,IHC. |
| 别名 | YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution.Positive control: Lane 1: HT29, Lane 2: Human skin, Lane 3: NIH/3T3. 2. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 antibody. Counter stained with hematoxylin. 3. Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle. 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon. 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10. Currently identified by mass spec as part of the TLR2 signaling complex and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 28 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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