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Alpha-Tubulin Loading Control Antibody (8C509)
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产品编号 TMAB-00108
Alpha-Tubulin Loading Control Antibody (8C509) 是一种 Mouse 抗体，靶向 Alpha-Tubulin Loading Control。Alpha-Tubulin Loading Control Antibody (8C509) 可用于 WB,IHC-P,IHC-Fr,IF。
Alpha-Tubulin Loading Control Antibody (8C509)
Alpha-Tubulin Loading Control Antibody (8C509)
还可以产品编号 TMAB-00108
Alpha-Tubulin Loading Control Antibody (8C509) 是一种 Mouse 抗体，靶向 Alpha-Tubulin Loading Control。Alpha-Tubulin Loading Control Antibody (8C509) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin Loading Control. Alpha-Tubulin Loading Control Antibody (8C509) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 8C509 |
| 交叉反应 | Human,Mouse,Rat,Zebrafish (predicted:Chicken,Dog,Pig,Rabbit,GuineaPig) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha Tubulin) Monoclonal Antibody, Unconjugated (ascites of TMAB-00108) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human HeLa cell lysates Lane 5: Human Jurkat cell lysates Lane 6: Human A431 cell lysates Lane 7: Human U251 cell lysates Lane 8: Human 293T cell lysates Lane 9: Human HepG2 cell lysates Primary: Anti-alpha Tubulin (TMAB-00108) at 1/100000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:50000-200000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
| 免疫原 | Recombinant Protein: human alpha Tubulin Protein |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Tubulin |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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