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Povidone iodine

产品编号 T3114 CAS 25655-41-8

别名: Betadine, 聚乙烯吡酮磺, PVP-I, PVP iodine, 聚乙烯吡咯烷酮碘, iodopovidone, Povidone-iodine, Isodine

Povidone iodine 有很强的抗细菌活性, 可杀死 MRSA 和 MSSA 菌株，其 MICs 值分别为 31.25 mg/L 和7.82 mg/L。

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Povidone iodine, CAS 25655-41-8

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生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	Povidone iodine displays excellent antibacterial activity which can against MRSA and MSSA strains with MICs of 31.25 mg/L and 7.82 mg/L, respectively.
体外活性	Exposure to PVP-I, of which concentrations are even lower than those used clinically, causes toxicity in epithelial cells[2].
体内活性	Povidone iodine is a broad spectrum antiseptic for topical application in the treatment and prevention of infection in wounds. A study investigating its use in defective tissue in neural structures reveals that Myelin changes are absent or minimal in all cases of the control group but are present as markedly increased myelin degeneration in nearly all cases in the PVP-I group. Axonal degeneration and hypoxic neuronal damage are absent in the control group, whereas they are marked in half of the PVP-I group. No statistically significant differences are established in Schwann cell proliferation, venous congestion, and lymphocytic proliferation between the two groups.
激酶实验	Kinase Inhibition Assay: Kinase inhibition is investigated using one of three assay formats: [33P]phosphoryl transfer, luciferase-coupled chemiluminescence, or AlphaScreen tyrosine kinase technology. IC50s are calculated by nonlinear regression analysis using XLFit.33P -Phosphoryl Transfer Kinase Assay Reactions are performed in 384-well white, clear bottom, high-binding microtiter plates (Greiner, Monroe, NC). Plates are coated with 2 μg/well of protein or peptide substrate in a 50 μL volume of coating buffer contained 40 μg/mL substrate (poly(Glu, Tyr) 4:1, 22.5 mM Na2CO3, 27.5 mM NaHCO3, 50 mM NaCl and 3 mM NaN 3. Coated plates are washed once with 50 μL of assay buffer following overnight incubation at room temperature (RT). Test compounds and enzymes are combined with 33P-γ-ATP (3.3 μCi/nmol) in a total volume of 20 μL. The reaction mixture is incubated at RT for 2 hours and terminated by aspiration. The microtiter plates are subsequently washed 6 times with 0.05% Tween-PBS buffer (PBST). Scintillation fluid (50 μL/well) is added and incorporated 33P is measured by liquid scintillation spectrometry using a MicroBeta scintillation counter.Luciferase-Coupled Chemiluminescence Assay Reactions are conducted in 384-well white, medium binding microtiter plates (Greiner). In a first step enzyme and compound are combined and incubated for 60 minutes; reactions are initiated by addition of ATP and peptide substrate (poly(Glu, Tyr) 4:1) in a final voume of 20 μL, and incubated at RT for 2-4 hours. Following the kinase reaction, a 20 μL aliquot of Kinase Glo (Promega, Madison, WI) is added and luminescence signal is measured using a Victor plate reader. Total ATP consumption is limited to 50%. AlphaScreenTM Tyrosine Kinase Assay Donor beads coated with streptavidin and acceptor beads coated with PY100 anti-phosphotyrosine antibody are used. Biotinylated poly(Glu,Tyr) 4:1 is used as the substrate.Substrate phosphorylation is measured by addition of donor/acceptor beads by luminescence following donor-acceptor bead complex formation. Kinase and test compounds are combined and preincubated for 60 minutes, followed by addition of ATP, and biotinylated poly(Glu, Tyr) in a total volume of 20 μL in 384-well white, medium binding microtiter plates (Greiner). Reaction mixtures are incubated for 1 hour at room temperature. Reactions are quenched by addition of 10 μL of 15-30 μg/mL AlphaScreen bead suspension containing 75 mM Hepes, pH 7.4, 300 mM NaCl, 120 mM EDTA, 0.3% BSA and 0.03% Tween-20. After 2-16 hours incubation at room temperature plates are read using an AlphaQuest reader.
细胞实验	One day after seeding, wells are exposed to PVP-I (0.01 to 1000 μM), which has been diluted with DMEM containing 10% FBS. Plates are further cultured for 1 or 2 days at 37℃ in a 5% CO2 incubator. The cell incubation time of 1 or 2 days is determined based on data regarding the onset time of iododerma and nephropathy pathogenesis.(Only for Reference)

别名	Betadine, 聚乙烯吡酮磺, PVP-I, PVP iodine, 聚乙烯吡咯烷酮碘, iodopovidone, Povidone-iodine, Isodine
分子量	364.95
分子式	C6H9I2NO
CAS No.	25655-41-8

存储

Powder: -20°C for 3 years | In solvent: -80°C for 2 years

溶解度

Ethanol: 27 mg/mL

DMSO: 27 mg/mL

H2O: 26 mg/mL

( < 1 mg/mL refers to the product slightly soluble or insoluble )

参考文献

1. Akcay E, et al. Childs Nerv Syst. 2012, 28(12):2071-5. 2. Sato S, et al. Drug Chem Toxicol. 2014, 37(3):268-75. 3. Wang Y, Yang T, Liu Y, et al. Decrease of miR-195 promotes chondrocytes proliferation and maintenance of chondrogenic phenotype via targeting FGF-18 pathway[J]. International journal of molecular sciences. 2017 May 4;18(5).

文献引用

1. Wang Y, Yang T, Liu Y, et al. Decrease of miR-195 promotes chondrocytes proliferation and maintenance of chondrogenic phenotype via targeting FGF-18 pathway. International Journal of Molecular Sciences. 2017 May 4;18(5) 2. Li D, Wang D, Cai J, et al.Notoginsenoside R1 facilitates cell angiogenesis by inactivating the Notch signaling during wound healing.Journal of Burn Care & Research.2023: irad035.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

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技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Povidone iodine 25655-41-8 Microbiology/Virology Antibacterial Antibiotic Betadine 聚乙烯吡酮磺 PVP-I PVP iodine 聚乙烯吡咯烷酮碘 iodopovidone Povidone-iodine Isodine inhibit Inhibitor Bacterial inhibitor

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Povidone iodine

存储

溶解度

参考文献

文献引用

相关产品

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剂量换算

体内实验配液计算器

计算器

输入分子式，点击计算，可计算出产品的分子量。

技术支持

Keywords