Powder: -20°C for 3 years | In solvent: -80°C for 1 year
N-Oleoyl glycine 是一种脂氨酸。在 3T3-L1 脂肪细胞中,N-Oleoyl glycine 通过激活CB1受体和Akt 信号通路来刺激脂肪形成。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 189 | 现货 | ||
5 mg | ¥ 421 | 现货 | ||
10 mg | ¥ 678 | 现货 | ||
25 mg | ¥ 1,130 | 现货 | ||
50 mg | ¥ 1,680 | 现货 | ||
100 mg | ¥ 2,480 | 现货 | ||
200 mg | ¥ 3,690 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 748 | 现货 |
产品描述 | N-Oleoyl glycine, a lipoamino acid, was able to promote 3T3-L1 adipogenesis through the activation of CB1 receptor and the enhancement of insulin-mediated Akt signaling pathway. |
体外活性 | N-Oleoyl glycine stimulated lipid accumulation and significantly increased adipogenic genes (PPARγ and aP2), in a dose- and time-dependent manner.?Additionally, N-Oleoyl glycine markedly increased the mRNA expression of CB1 receptor and the inhibition of CB1R by its antagonist SR141716 abolished the promotive effects of N-Oleoyl glycine on lipid accumulation and the protein expression of PPARγ and aP2.?Furthermore, N-Oleoyl glycine increased the ratio of p-Akt/Akt and p-FoxO1/FoxO1, which could be reversed by SR141716.?Moreover, N-Oleoyl glycine-induced enhancement of adipogenesis, activation of insulin-mediated Akt signaling pathway and inactivation of FoxO1 were effectively blocked by Wortmannin, a specific PI3K/Akt inhibitor, indicating the essential role of Akt signaling pathway in the process of N-Oleoyl glycine-stimulated 3T3-L1 adipogenesis[1]. |
分子量 | 339.51 |
分子式 | C20H37NO3 |
CAS No. | 2601-90-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 80 mg/mL (235.63 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.9454 mL | 14.7271 mL | 29.4542 mL | 73.6355 mL |
5 mM | 0.5891 mL | 2.9454 mL | 5.8908 mL | 14.7271 mL | |
10 mM | 0.2945 mL | 1.4727 mL | 2.9454 mL | 7.3636 mL | |
20 mM | 0.1473 mL | 0.7364 mL | 1.4727 mL | 3.6818 mL | |
50 mM | 0.0589 mL | 0.2945 mL | 0.5891 mL | 1.4727 mL | |
100 mM | 0.0295 mL | 0.1473 mL | 0.2945 mL | 0.7364 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
N-Oleoyl glycine 2601-90-3 Cytoskeletal Signaling DNA Damage/DNA Repair GPCR/G Protein Metabolism PI3K/Akt/mTOR signaling Cannabinoid Receptor Akt Endogenous Metabolite PPAR Inhibitor NOleoyl glycine Protein kinase B N Oleoyl glycine PKB inhibit inhibitor