keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Calcein-AM is a cell-permeable fluorescent dye used for the determination of cell viability.
产品描述 | Calcein-AM is a cell-permeable fluorescent dye used for the determination of cell viability. |
体外活性 | The calcein-AM dye used to stain the living cells have a low spontaneous leakage rate of less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM have a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this micro-test. Quantitation of killing and kinetic analysis is readily performed with the test system [1]. Calcein-AM is pH-independent, better retained, and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader [2]. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with the intact plasma membrane. The calcein-AM assay has been used to assess cell viability, cytotoxicity, and tp quantitate apoptosis [3]. |
别名 | Calcein acetoxymethyl ester, 钙黄绿素-AM |
分子量 | 994.86 |
分子式 | C46H46N2O23 |
CAS No. | 148504-34-1 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Calcein-AM 148504-34-1 Others Calcein acetoxymethyl ester CalceinAM 钙黄绿素-AM Calcein AM Inhibitor inhibitor inhibit