store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
5-IAF 是吲哚乙酰胺的一种荧光素衍生物,是一种荧光探针,可用来标记蛋白质和其他含有游离硫醇(半胱氨酸侧链)。5-IAF 可标记狗肾脏Na,K-ATP 酶的催化(α)亚单位。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 252 | 现货 | ||
5 mg | ¥ 611 | 现货 | ||
10 mg | ¥ 958 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 792 | 现货 |
产品描述 | 5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains). 5-IAF can label the catalytic (alpha) subunit of dog kidney Na,K-ATPase. |
体外活性 | 8 µM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 µM 5-IAF, respectively. It was analyzed with CE-LIF, the highest signal intensity is obtained with 400 and 800 µM 5-IAF.[2] |
别名 | 5-Iodoacetamidofluorescein |
分子量 | 515.25 |
分子式 | C22H14INO6 |
CAS No. | 63368-54-7 |
store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMF: 90.0 mg/mL (174.7 mM ), Sonication and heating to 60℃ are recommended.
DMSO: 45.0 mg/mL (87.3 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMF / DMSO | 1 mM | 1.9408 mL | 9.704 mL | 19.4081 mL | 48.5201 mL |
5 mM | 0.3882 mL | 1.9408 mL | 3.8816 mL | 9.704 mL | |
10 mM | 0.1941 mL | 0.9704 mL | 1.9408 mL | 4.852 mL | |
20 mM | 0.097 mL | 0.4852 mL | 0.9704 mL | 2.426 mL | |
50 mM | 0.0388 mL | 0.1941 mL | 0.3882 mL | 0.9704 mL | |
DMF | 100 mM | 0.0194 mL | 0.097 mL | 0.1941 mL | 0.4852 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
5-IAF 63368-54-7 5-Iodoacetamidofluorescein Inhibitor inhibitor inhibit