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CTLA-4 Protein, Human, Recombinant (Flag)

CTLA-4 Protein, Human, Recombinant (Flag)

产品编号 TMPJ-00107
别名: Cytotoxic T-lymphocyte protein 4, CTLA-4, Cytotoxic T-lymphocyte-associated antigen 4, CD152, CTLA4

CTLA-4 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 20 KDa and the accession number is P16410.

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CTLA-4 Protein, Human, Recombinant (Flag)
规格 价格/CNY 货期 数量
10 μg ¥ 220 5日内发货
50 μg ¥ 700 5日内发货
500 μg ¥ 4,740 5日内发货
1 mg ¥ 7,510 5日内发货
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产品目录号及名称: CTLA-4 Protein, Human, Recombinant (Flag) (TMPJ-00107)
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产品描述 CTLA-4 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 20 KDa and the accession number is P16410.
种属 Human
表达系统 HEK293 Cells
标签 C-Flag
蛋白编号 P16410
别名 Cytotoxic T-lymphocyte protein 4, CTLA-4, Cytotoxic T-lymphocyte-associated antigen 4, CD152, CTLA4
氨基酸序列 Lys36-Asp161
蛋白构建 Lys36-Asp161
蛋白纯度 Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量 20 KDa (reducing condition)
内毒素 < 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.4.
复溶方法 Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

研究背景 Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

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Keywords

CTLA-4 Protein, Human, Recombinant (Flag) Cytotoxic T-lymphocyte protein 4 CD 152 CTLA-4 CTLA 4 Cytotoxic T-lymphocyte-associated antigen 4 CD-152 CD152 CTLA4 recombinant recombinant-proteins proteins protein

 

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