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CTLA-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15 kDa and the accession number is P16410-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 1,630 | 现货 |
生物活性 | 1. Immobilized Recombinant Human CD86/B7-2 Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (His Tag) , the EC50 is 2.6-8 ng/mL. _x000D_ 2. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3 μg/mL when stimulated with 1 μg/mL Recombinant Human B7‑1/CD80. _x000D_ 3. Captured Ipilimumab on proA Chip can bind CTLA-4 with an affinity constant of 20.03 nM as determined in a SPR assay (Routinely tested). _x000D_ 4. Loaded Ipilimumab on ProA Biosensor, can bind CTLA-4 Protein, Human, Recombinant, His Tag with an affinity constant of 5.15 nM as determined in BLI assay (Routinely tested). ![]() ![]() ![]() ![]() |
产品描述 | CTLA-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15 kDa and the accession number is P16410-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | P16410-1 |
别名 | IDDM12,GSE,GRD4,cytotoxic T-lymphocyte-associated protein 4,CTLA-4,CELIAC3,CD152,CD,ALPS5 |
蛋白构建 | A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the a polyhistidine tag at the C-terminus. Predicted N terminal: Lys 36 |
蛋白纯度 | > 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ![]() ![]() |
分子量 | 15 kDa (predicted); 22-27 kDa (reducing condition, due to glycosylation) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsCTLA4 / CD152 Immune Checkpoint ProteinsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |