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PCNA Nuclear Loading Control Polyclonal Antibody

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PCNA Nuclear Loading Control Polyclonal Antibody
产品编号 TMAB-01343

PCNA Nuclear Loading Control Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PCNA Nuclear Loading Control。PCNA Nuclear Loading Control Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

PCNA Nuclear Loading Control Polyclonal Antibody

PCNA Nuclear Loading Control Polyclonal Antibody

Rating icon 还可以
PCNA Nuclear Loading Control Polyclonal Antibody
产品编号 TMAB-01343

PCNA Nuclear Loading Control Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PCNA Nuclear Loading Control。PCNA Nuclear Loading Control Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

规格价格库存数量
50 μL
¥ 670
5日内发货
100 μL
¥ 960
5日内发货
500 μL
¥ 3,995
5日内发货
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产品介绍

生物活性
产品描述
PCNA Nuclear Loading Control Polyclonal Antibody is a Rabbit antibody targeting PCNA Nuclear Loading Control. PCNA Nuclear Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. 25 μg total protein per Lane of various lysates probed with PCNA polyclonal antibody, unconjugated (TMAB-01343) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
18. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
19. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
20. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
应用
WB
IHC-P
IHC-Fr
ICC/IF
IF
FCM
推荐剂量
WB: 1:1000-10000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; ICC/IF: 1:100-500; IF: 1:200-1000; FCM: 1ug/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Nucleus. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.
抗原信息
免疫原
KLH conjugated synthetic peptide: mouse PCNA
抗原种属
Mouse
基因ID
18538
研究领域
Cell division,Tumor antigens,Markers,DNA / RNA binding,DNA Synthesis,Methylated DNA Binding,PCNA,Replication
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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