Anti-THRB Polyclonal Antibody is a Rabbit antibody targeting THRB. Anti-THRB Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM,ELISA.

IgG

Human

1. Measured by its binding ability in a indirect ELISA. Immobilized Human THRB protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human THRB Antibody, the EC50 is 133.9 ng/mL.
2. Measured by its binding ability in a indirect ELISA. Immobilized Human THRB protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human THRB Antibody, the EC50 is 942.9 ng/mL.
3. Blank control (black line): A431.
Primary Antibody (green line): Rabbit Anti-THRB antibody (TMAB-13516)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
4. Blank control (black line): A431.
Primary Antibody (green line): Rabbit Anti-THRB antibody (TMAB-13516)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
5. Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRB) Polyclonal Antibody, Unconjugated (TMAB-13516) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (THRB) Polyclonal Antibody, Unconjugated (TMAB-13516) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-THRB antibody (TMAB-13516)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
8. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-THRB antibody (TMAB-13516)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

IHC-P: 1:200-800; IHC-Fr: 1:400-800; IF: 1:100-500; FCM: 1μg/Test; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: human THRB protein

Human

THRB

Thyroid hormone receptor beta

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.