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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-RAP80 Antibody (7N70)

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货号 TMAB-12089

Anti-RAP80 Antibody (7N70) 是一种 Rabbit 抗体，靶向 RAP80。Anti-RAP80 Antibody (7N70) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

Anti-RAP80 Antibody (7N70)

Anti-RAP80 Antibody (7N70)

一键复制产品信息
Rating icon 还可以
货号 TMAB-12089

Anti-RAP80 Antibody (7N70) 是一种 Rabbit 抗体，靶向 RAP80。Anti-RAP80 Antibody (7N70) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

规格价格库存数量
25 μL
¥ 785
5日内发货
50 μL
¥ 1,390
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-RAP80 Antibody (7N70) is a Rabbit antibody targeting RAP80. Anti-RAP80 Antibody (7N70) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
克隆号
7N70
反应种属
Human
验证活性
1. 25 μg total protein per lane of various lysates (see on figure) probed with RAP80 monoclonal antibody, unconjugated (TMAB-12089) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-RAP80 antibody (TMAB-12089, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
应用WBIHC-PIHC-FrICC/IFIFFCM
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
研究背景Ubiquitin-binding protein.Specifically recognizes and binds 'Lys-63'-linked ubiquitin.Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs).
抗原信息
免疫原
A synthesized peptide: human RAP80
抗原种属
Human
基因名称
UIMC1
基因ID
51720
蛋白名称
BRCA1-A complex subunit RAP80
Uniprot ID
Q96RL1
化学信息
分子量Theoretical: 80 kDa. Actual: 80 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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