Anti-NEFM Antibody (2I590) is a Mouse antibody targeting NEFM. Anti-NEFM Antibody (2I590) can be used in WB,IHC-P,IF,IHC-Fr.

IgG1

2I590

Mouse,Rat,Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with NEFM monoclonal antibody, unconjugated (TMAB-09339) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
9. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NEFM Monoclonal Antibody, Unconjugated (TMAB-09339) at 1: 200 overnight at 4°C, followed by conjugation to the bs-0296G-HRP and DAB staining and DAB staining.

WB: 1: 500- 2000; IHC-P: 1: 200-500; IF: 1: 200-500; IHC-Fr: 1: 200-500

Monoclonal

Recombinant Protein: Mouse NF-M protein

Mouse

NEFM

Neurofilament medium polypeptide

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.