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Anti-MYL3 Antibody (5Q450)

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货号 TMAB-09157

Anti-MYL3 Antibody (5Q450) 是一种 Mouse 抗体，靶向 MYL3。Anti-MYL3 Antibody (5Q450) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-MYL3 Antibody (5Q450)

Anti-MYL3 Antibody (5Q450)

一键复制产品信息
Rating icon 还可以
货号 TMAB-09157

Anti-MYL3 Antibody (5Q450) 是一种 Mouse 抗体，靶向 MYL3。Anti-MYL3 Antibody (5Q450) 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
25 μL
¥ 780
5日内发货
50 μL
¥ 1,390
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-MYL3 Antibody (5Q450) is a Mouse antibody targeting MYL3. Anti-MYL3 Antibody (5Q450) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG1, Kappa
克隆号
5Q450
反应种属
Human,Mouse,Rat
验证活性
1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: Mouse heart, Rat heart
Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted MW: 22-25 kDa
Observed MW: 22-25 kDa
2. Tissue: Human skeletal muscle
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:50
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-09157
3. Tissue: Rat heart
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:50
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-09157
4. Tissue: Mouse skeletal muscle
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:50
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-09157
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:1000-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Monoclonal
宿主来源Mouse
构建方式Recombinant Antibody
纯化方式Protein G purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.
抗原信息
基因名称
MYL3
基因ID
4634
蛋白名称
Myosin light chain 3
功能
Regulatory light chain of myosin. Does not bind calcium.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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