Anti-MKI67 Antibody (9T614) is a Mouse antibody targeting MKI67. Anti-MKI67 Antibody (9T614) can be used in FCM.

IgG

9T614

Human

Blank control: Hela.
Primary Antibody (green line): Mouse Anti-Ki-67 antibody (TMAB-01143)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 1ug/Test

Monoclonal

KLH conjugated synthetic peptide: human Ki-67

Human

Cell division,Tumor biomarkers,Markers,Soma marker,Neurogenesis,Replication