Anti-LAMP1 Antibody (2T47) is a Rabbit antibody targeting LAMP1. Anti-LAMP1 Antibody (2T47) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

2T47

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (TMAB-08210) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (TMAB-08210) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (TMAB-08210) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (TMAB-08210) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (TMAB-08210) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (TMAB-08210) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (TMAB-08210) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP1 Monoclonal Antibody, Unconjugated (TMAB-08210) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. 25 ug total protein per lane of various lysates (see on figure) probed with LAMP1 monoclonal antibody, unconjugated (TMAB-08210) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
10. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (LAMP1) monoclonal Antibody, unconjugated (TMAB-08210) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

ICC/IF=1:50-200; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:1000-5000

Monoclonal

Recombinant Protein: human LAMP1 protein

Human

LAMP1

Lysosome-associated membrane glycoprotein 1

Presents carbohydrate ligands to selectins. Also mplicated in tumor cell metastasis.