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Anti-human CD90/Thy-1 Antibody (4K978) 是一种 Mouse 抗体,靶向 human CD90/Thy-1。Anti-human CD90/Thy-1 Antibody (4K978) 可用于 FCM。
Anti-human CD90/Thy-1 Antibody (4K978) 是一种 Mouse 抗体,靶向 human CD90/Thy-1。Anti-human CD90/Thy-1 Antibody (4K978) 可用于 FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 1,865 | 5日内发货 |
| 产品描述 | Anti-human CD90/Thy-1 Antibody (4K978) is a Mouse antibody targeting human CD90/Thy-1. Anti-human CD90/Thy-1 Antibody (4K978) can be used in FCM. |
| Ig Type | Mouse IgG1 |
| 克隆号 | 4K978 |
| 反应种属 | Human |
| 验证活性 | Blank control: HepG2. Primary Antibody (green line): Mouse Anti-CD90 antibody (TMAB-07423) Dilution: 2 μg /10^6 cells; Isotype Control Antibody (orange line): Mouse IgG. Secondary Antibody: Goat anti-Mouse IgG-CY5 Dilution: 1 μg /test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| 应用 | FCM |
| 推荐剂量 | FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 组织特异性 | Abundant in lymphoid tissues. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M PBS (pH7.4). |
| 浓度 | 0.5mg/ml |
| 研究背景 | Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation. |
| 免疫原 | HEL cells |
| 基因名称 | THY1 |
| 基因ID | |
| 蛋白名称 | Thy-1 membrane glycoprotein |
| 功能 | May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. |
| 分子量 | Theoretical: 12 kDa. Actual: 25-35 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |