Anti-HMGN2/HMG17 Antibody (7I909) is a Rabbit antibody targeting HMGN2/HMG17. Anti-HMGN2/HMG17 Antibody (7I909) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

7I909

Human,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with HMGN2/HMG17 monoclonal antibody, unconjugated (TMAB-07191) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HMGN2/HMG17 Monoclonal Antibody, Unconjugated (TMAB-07191) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HMGN2/HMG17 Monoclonal Antibody, Unconjugated (TMAB-07191) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HMGN2/HMG17 Monoclonal Antibody, Unconjugated (TMAB-07191) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HMGN2/HMG17 Monoclonal Antibody, Unconjugated (TMAB-07191) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HMGN2/HMG17 Monoclonal Antibody, Unconjugated (TMAB-07191) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HMGN2/HMG17 Monoclonal Antibody, Unconjugated (TMAB-07191) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HMGN2/HMG17 Monoclonal Antibody, Unconjugated (TMAB-07191) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei.
9. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-HMGN2/HMG17 antibody (TMAB-07191, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
10. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HMGN2/HMG17) monoclonal Antibody, unconjugated (TMAB-07191) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

A synthesized peptide: human HMGN2

Human

HMGN2

Non-histone chromosomal protein HMG-17

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation.