Anti-GOLPH2 Polyclonal Antibody is a Rabbit antibody targeting GOLPH2. Anti-GOLPH2 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Cow,Horse)

Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-GOLPH2 antibody (TMAB-06635)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GOLPH2

Human

GOLM1

Golgi membrane protein 1

Unknown. Cellular response protein to viral infection.