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Anti-GATA4 Antibody (5B64)

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货号 TMAB-06359

Anti-GATA4 Antibody (5B64) 是一种 Rabbit 抗体，靶向 GATA4。Anti-GATA4 Antibody (5B64) 可用于 WB,ICC/IF,FCM。

Anti-GATA4 Antibody (5B64)

Anti-GATA4 Antibody (5B64)

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Rating icon 还可以
货号 TMAB-06359

Anti-GATA4 Antibody (5B64) 是一种 Rabbit 抗体，靶向 GATA4。Anti-GATA4 Antibody (5B64) 可用于 WB,ICC/IF,FCM。

规格价格库存数量
25 μL
¥ 785
5日内发货
50 μL
¥ 1,385
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100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-GATA4 Antibody (5B64) is a Rabbit antibody targeting GATA4. Anti-GATA4 Antibody (5B64) can be used in WB,ICC/IF,FCM.
Ig Type
IgG
克隆号
5B64
反应种属
Human,Mouse,Rat
验证活性
1. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (GATA4) monoclonal Antibody, unconjugated (TMAB-06359) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with GATA4 monoclonal antibody, unconjugated (TMAB-06359) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
应用WBICC/IFFCM
推荐剂量
WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Nucleus.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
研究背景Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function.In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression.
抗原信息
免疫原
A synthesized peptide: human GATA4
抗原种属
Human
基因名称
GATA4
基因ID
2626
蛋白名称
Transcription factor GATA-4
Uniprot ID
P43694
功能
Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement.
化学信息
分子量Theoretical: 45 kDa. Actual: 54 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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