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Anti-CD3D Antibody (4C442) 是一种 Mouse 抗体，靶向 CD3D。Anti-CD3D Antibody (4C442) 可用于 IHC-P。
还可以Anti-CD3D Antibody (4C442) 是一种 Mouse 抗体，靶向 CD3D。Anti-CD3D Antibody (4C442) 可用于 IHC-P。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,689 | 5日内发货 |
| 产品描述 | Anti-CD3D Antibody (4C442) is a Mouse antibody targeting CD3D. Anti-CD3D Antibody (4C442) can be used in IHC-P. |
| 别名 | T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex), CD3 δ, CD3 delta |
| Ig Type | Mouse IgG1 |
| 克隆号 | 4C442 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human CD3D in human tonsil with mouse Mab (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of T cells. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein). 2. Immunochemical staining of human CD3D in human spleen with mouse Mab (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of T cells. |
| 应用 | IHC-P |
| 推荐剂量 | IHC-P: 1:50-1:200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3d / CD3 delta (rh CD3d / CD3 delta; TMPY-01196; NP_000723.1; Met 1-Ala 105). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCR alpha and beta chains along with invariant CD3 gamma, delta, epsilon, and zeta chains. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3 delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCR gamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human CD3d / CD3 delta protein (TMPY-01196) |
| 抗原种属 | Human |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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