Anti-CAPG2 Polyclonal Antibody is a Rabbit antibody targeting CAPG2. Anti-CAPG2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Sample:
293T (Human) Cell Lysate at 30 μg
Primary: Anti-CAPG2 (TMAB-03606) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD
2. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CAPG2 antibody (TMAB-03606)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human A431 cell lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human U251 cell lysates
Lane 7: Human Jurkat cell lysates
Primary: Anti-CAPG2 (TMAB-03606) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa
4. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CAPG2) Polyclonal Antibody, Unconjugated (TMAB-03606) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CAPG2/NCAPG2

Human

NCAPG2

Condensin-2 complex subunit G2

Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.