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Anti-Aminopeptidase N/CD13 Antibody (3U499) 是一种 Rabbit 抗体,靶向 Aminopeptidase N/CD13。Anti-Aminopeptidase N/CD13 Antibody (3U499) 可用于 WB,ICC,IHC,IP。
Anti-Aminopeptidase N/CD13 Antibody (3U499) 是一种 Rabbit 抗体,靶向 Aminopeptidase N/CD13。Anti-Aminopeptidase N/CD13 Antibody (3U499) 可用于 WB,ICC,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-Aminopeptidase N/CD13 Antibody (3U499) is a Rabbit antibody targeting Aminopeptidase N/CD13. Anti-Aminopeptidase N/CD13 Antibody (3U499) can be used in WB,ICC,IHC,IP. |
别名 | PEPN, p150, LAP1, GP150, CD13, APN, alanyl (membrane) aminopeptidase |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution. Positive control: Lane 1: THP-1, Lane 2: human heart Lane 3: U937. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD13 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD13 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD13 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD13 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD13 antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CD13 antibody. Counter stained with hematoxylin. 8. ICC staining CD13 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining CD13 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining CD13 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:100-500 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | CD13, or aminopeptidase N, is a type II transmembrane glycoprotein that is expressed on most cells of Myeloid origin, including monocytes, basophils, eosinophils, neutrophils and Myeloid leukemias. CD13 is also found on certain epithelial cells, fibroblasts and osteoclasts. CD13 acts as a zinc-binding metalloprotease that plays a role in digestion and may function in the inactivation of some regulatory peptides such as enkephalins. CD13 may play a role in the invasion of cancer cells by enhancing their invasive capacity and metastatic behavior. The activity of CD13 can be inactivated using specific inhibitors that evoke apoptosis of CD13-positive cancer cells. Basic fibroblast growth factor (bFGF) expression upregulates CD13 expression in human melanoma cells by activating both the Myeloid and the epithelial CD13 promoter. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 150 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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