Anti-AER61 Antibody (2H595) is a Rabbit antibody targeting AER61. Anti-AER61 Antibody (2H595) can be used in FCM, WB.

IgG

2H595

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with AER61 monoclonal antibody, unconjugated (TMAB-02369) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 25 ug total protein per lane of various lysates (see on figure) probed with AER61 monoclonal antibody, unconjugated (TMAB-02369) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. The U-87MG (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-AER61 antibody (TMAB-02369, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; WB=1:500-2000

Monoclonal

A synthesized peptide: human EOGT

Human

EOGT

EGF domain-specific O-linked N-acetylglucosamine transferase

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains (By similarity).