photoaffinity

Nitrobenzylthioinosine (NBMPR) 是一种 ENT1 转运蛋白抑制剂，可与 ENT1 转运蛋白以高亲和力结合。它是一种可穿过血脑屏障的光亲和探针，用于追踪大脑中腺苷的摄取位点。

YM 534 具有抗肿瘤活性和血小板凝集活性，抑制去糖基化P-糖蛋白的光亲和标记，可用于研究白血病。

Benzoylphenylalanine (LBPA) 可用作光亲和肽灭活剂。

PARPYnD is a potent photoaffinity probe for poly(ADP-ribose) polymerase (PARP) (IC50 values for PARP2, PARP1 and PARP6 are 6, 38 and 230 nM, respectively). PARPYnD labels PARP1 and PARP2 in the cell when an N3 functionalized fluorescent probe is attached and can inhibit isolated PARP6.

GNF351 是芳烃受体 (AhR) 的完全拮抗剂，具有抑制 DRE 依赖性和非依赖性活性的能力。它与结合到 AhR 的光亲和性 AhR 配体竞争，IC50为 62 nM。它在小鼠或人角质形成细胞中的毒性极小。

4-Azido-L-phenylalanine (p-Azido-L-phenylalanine) 是一种氨基酸衍生物，可作为一种光亲和标记，可用于检测局部蛋白质环境。

L 669083 is two classes of potent leukotriene biosynthesis inhibitors photoaffinity analogue.

L458-BPyne is a γ-secretase modulator photoaffinity probe that acts by identifying distinct allosteric binding sites on presenilin. It also can be exploited to reduce levels of pathogenic Aβ species.

RO-57-Bpyne is a γ-secretase modulator photoaffinity probe. It also can be exploited to reduce levels of pathogenic Aβ species. It acts by identifying distinct allosteric binding sites on presenilin.

163-bp3 is an effective photoaffinity probe and can be used as a target γ Secretory enzyme.

BI-RJ 70 is a tritiated photoaffinity azide analog of nevirapine, which can be used to study the binding of nevirapine to HIV-1 transcriptase through covalently labeled enzymes and amino acid residues.

CGP 55802A is a novel photoaffinity ligand for in-situ labeling of NMDA receptors.

LU 49888 is a photoaffinity analog of verapamil that has been used to identify specific binding sites for phenylalkylamines of calcium channels present in rabbit skeletal muscle microsomes.

2,6-Dichloropurine-9-β-D-riboside is a building block.1,2It has been used in the synthesis of photoaffinity probes for nucleotide binding sites in proteins. 1.Wower, J., Hixson, S.S., Sylvers, L.A., et al.Synthesis of 2,6-diazido-9-(β-D-ribofuranosyl)purine 3',5'-bisphosphate: Incorporation into transfer RNA and photochemical labeling of Escherichia coli ribosomesBioconjug. Chem.5(2)158-161(1994) 2.Seiter, M.S., Bauer, M.P., Bogel, P.D., et al.Synthesis of novel spin-labeled photoaffinity derivatives of NAD+ and ATP and their characterization as coenzymes and substrates of several enzymesSynthesis2269-273(1996)

Azidothiorphan is a photoaffinity ligand for the active site of the neutral endopeptidase 24.11. After ultraviolet irradiation the inhibitor binds irreversibly to the enzyme, and photolabeling occurs at the active site. The competitive inhibitor thiorphan protects the endopeptidase from inactivation.

Antibacterial agent 116 是一种含二苯甲酮的水杨酰苯胺类化合物。Antibacterial agent 116 能抑制肽聚糖的形成而具有较强的抗菌活性。Antibacterial agent 116 可作为光亲和探针标记 Acinetobacter baumannii 的青霉素结合蛋白 (PBP1b)，干扰肽聚糖链的生长，阻断其增殖。

Fmoc-L-photo-proline为可通过Fmoc固相法纳入合成肽中的光交联氨基酸。该化合物在环状拟肽抗生素合成及肽基光亲和探针研究制备中应用广泛。

4-Azido-2,3,5,6-tetrafluorobenzoic Acid (N3-TFBA) 作为一种含叠氮基的点击化学试剂，常被应用于标记法中。该化合物是带有 FAM 标记的 DNA 探针的重要组成部分，同时也能作为多功能光亲和标记剂用于生物受体的探测。

Fmoc-L-Lys(4-N3-Z)-OH 是一种具备叠氮基功能的化学试剂,并作为SPPS中的赖氨酸构建块,用于生物正交连接、红外探针及光亲和试剂.此化合物通过细菌诱导的1,3-偶极环加成反应,可以进行反式环辛烯醇脱羧.作为点击化学工具,Fmoc-L-Lys(4-N3-Z)-OH 含有Azide基团,能与含Alkyne基团的分子通过铜催化的叠氮-炔环加成反应(CuAAc)产生作用,亦可与含DBCO或BCN基团的分子进行细菌诱导的炔-叠氮环加成反应(SPAAC).

Nph-peptide can be used for photoaffinity labeling of the N-formyl peptide receptor site of intact human polymorphonuclear leukocytes.