antifungal agent 20

Antifungal agent 20 demonstrates significant antifungal efficacy against a range of fungal pathogens including Colletotrichum gloeosprioides, Rhizoctonia solani, Phytophthora nicotianae var. nicotianae, Diplodia pinea, Colletotrichum acutatum, and Fusarium oxysporum f. sp. niveum.

CYP51-IN-20 (compound 5b) 作为CYP51的有效抑制剂，显示了对白色念珠菌 ATCC 10231 的出色抗真菌效果。该化合物不仅下调ERG11(Cyp51) 基因表达，还显著地阻止了酵母到菌丝的形态转变。此外，CYP51-IN-20 与Voriconazole 共同作用，能有效占据CYP51的完整结合位点，在蜡螟模型中展示了其协同的抑制效能。

SDH-IN-20 (compound A19) 是一种抗真菌剂，其对Verticillium dahlia和R. solani的EC50值分别低于3.0 μg mL和2.87 μg mL。作为一种高效的琥珀酸脱氢酶 (SDH) 抑制剂，SDH-IN-20 显示出强效的抗真菌活性，其IC50值为29.33 μM。此外，SDH-IN-20 显著破坏了R. solani的细胞膜完整性和菌丝形态。

Heronamide C, a polyketide macrolactam antifungal agent synthesized by marine Actinomycetes, exhibits selective toxicity by inhibiting growth and promoting abnormal cell wall material accumulation in wild-type fission yeast cells (MIC = 0.13-0.28 μM), without affecting Gram-positive bacteria or human cancer cell lines, HeLa and MDA-MB-231. At 20 μM, it triggers reversible morphological alterations, notably the development of large intracellular structures, in HeLa cells.