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PAM Protein, Human, Recombinant (His)

产品编号 TMPY-00392

PAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 94.4 kDa and the accession number is P19021-2.

PAM Protein, Human, Recombinant (His)

PAM Protein, Human, Recombinant (His)

产品编号 TMPY-00392
PAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 94.4 kDa and the accession number is P19021-2.
规格价格库存数量
50 μg¥ 4,470现货
100 μg¥ 7,6305日内发货
200 μg¥ 13,0105日内发货
500 μg¥ 26,2905日内发货
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生物活性

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
PAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 94.4 kDa and the accession number is P19021-2.
种属
Human
表达系统
HEK293 Cells
标签C-His
蛋白编号P19021-2
别名
PAL,peptidylglycine alpha-amidating monooxygenase,peptidylglycine α-amidating monooxygenase,PHM
蛋白构建
A DNA sequence encoding the human PAM (NP_620176.1) (Met1-Ser866) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Phe 31
蛋白纯度
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
PAM Protein, Human, Recombinant (His)PAM Protein, Human, Recombinant (His)
分子量94.4 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Peptidylglycine alpha-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs). Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides.

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