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IL-23 Protein, Mouse, Recombinant (His)
Synonyms: 白细胞介素, 白介素, p19, IL-23货号 TMPY-03526 一键复制产品信息纯度: 96%
很棒
很棒SPR兼容缓冲液
26年新产品线折扣促销活动
IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432.
IL-23 Protein, Mouse, Recombinant (His)
一键复制产品信息 很棒SPR兼容缓冲液
26年新产品线折扣促销活动
纯度: 96%
货号 TMPY-03526
别名 白细胞介素, 白介素, p19, IL-23
IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432.
其他形式的 “IL-23 Protein, Mouse, Recombinant (His)”:
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 458 | 现货 | |
| 10 μg | ¥ 743 | 现货 | |
| 20 μg | ¥ 1,230 | 3日内发货 | |
| 50 μg | ¥ 2,430 | 现货 | |
| 100 μg | ¥ 4,310 | 3日内发货 | |
| 200 μg | ¥ 7,620 | 3日内发货 | |
| 500 μg | ¥ 16,100 | 现货 |
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产品介绍
生物活性
化学信息
储存&溶解度
| 产品描述 | IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432. |
| 生物活性 | 1. Immobilized Human IL23 Receptor hFc at 2 μg/ml (100 μl/well) can bind Mouse IL-23 His, the EC50 of Mouse IL-23 His is 50-250 ng/mL.
2. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-1 ng/mL.  |
| 种属 | Mouse |
| 表达系统 | HEK293 Cells |
| 标签 | C-His |
| 蛋白编号 | Q9EQ14&P43432 |
| 蛋白构建 | A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified. Predicted N terminal: Val 22 & Met 23 |
| 蛋白纯度 | > 95 % as determined by SDS-PAGE  |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstituted with sterile deionized water to 0.25 mg/mL. |
| 疾病造模方案 | 构建银屑病样皮肤炎症模型
*注意事项：造模后 3 天或 7 天处死小鼠，快速分离背部皮肤、血浆样本，皮肤组织部分固定于福尔马林（组织学检测），部分冻存用于蛋白及 RNA 提取。 |
| 别名 | 白细胞介素, 白介素, p19, IL-23 |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 分子量 | 58.3 kDa (predicted); 47,45 and 26 kDa (reducing conditions) |
| 运输方式 | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. 实际储存温度请以COA为准 |
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