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IL-23 Protein, Mouse, Recombinant (His)

TargetMol | SPRIL-23 Protein, Mouse, Recombinant (His)
产品编号 TMPY-03526
IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432.
规格价格库存数量
50 μg
¥ 2,430
In stock
100 μg
¥ 4,310
5日内发货
200 μg
¥ 7,620
5日内发货
500 μg
¥ 16,130
In stock
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产品信息

生物活性
1. Immobilized Human IL23 Receptor hFc at 2 μg/ml (100 μl/well) can bind Mouse IL-23 His, the EC50 of Mouse IL-23 His is 50-250 ng/mL. 2. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-1 ng/mL.
IL-23 Protein, Mouse, Recombinant (His)
产品描述
IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432.
种属
Mouse
表达系统
HEK293 Cells
标签C-His
蛋白编号Q9EQ14&P43432
别名
白细胞介素,白介素,p19,IL-23
蛋白构建
A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified. Predicted N terminal: Val 22 & Met 23
蛋白纯度
> 95 % as determined by SDS-PAGE
IL-23 Protein, Mouse, Recombinant (His)
分子量58.3 kDa (predicted); 47,45 and 26 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
Reconstituted with sterile deionized water to 0.25 mg/mL.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.

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