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HMGB1 Protein, Human, Recombinant (hFc)

产品编号 TMPY-00822

别名: HMG1, SBP-1, high mobility group box 1, HMG3, HMG-1

HMGB1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 51.5 kDa and the accession number is A0A024RDR0.

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HMGB1 Protein, Human, Recombinant (hFc)

规格	价格/CNY		货期	数量
50 μg	￥ 3,170		5日内发货
500 μg	￥ 16,500		5日内发货

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生物活性

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参考文献

生物活性	Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse AGER at 2 μg/ml (100 μl/well) can bind human HMGB1. The EC50 of human HMGB1 is 0.23 μg/ml.
产品描述	HMGB1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 51.5 kDa and the accession number is A0A024RDR0.

种属	Human
表达系统	HEK293 Cells
标签	N-hFc
蛋白编号	A0A024RDR0
别名	HMG1, SBP-1, high mobility group box 1, HMG3, HMG-1
蛋白构建	The Human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was expressed with the fused Fc region of Human IgG1 at the N-terminus.
蛋白纯度	≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	51.5 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2, and HMGB3. HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. It is the prototypic damage-associated molecular pattern (DAMP) molecule and has been implicated in several inflammatory disorders. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis are present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses. Extracellular HMGB1 represents an optimal " necrotic marker" selected by the innate immune system to recognize tissue damage and initiate reparative responses. However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis. As shown in studies on patients as well as animal models, HMGB1 can play an important role in the pathogenesis of the rheumatic disease, including rheumatoid arthritis, systemic lupus erythematosus, and polymyositis among others. Besides, enhanced postmyocardial infarction remodeling in type 1 diabetes mellitus was partially mediated by HMGB1 activation.

参考文献

1. Ulloa L,et al.(2006) High-mobility group box 1 (HMGB1) protein: friend and foe. Cytokine Growth Factor Rev. 17 (3): 189-201. 2. Pisetsky DS,et al.(2008) High-mobility group box protein 1 (HMGB1): an alarmin mediating the pathogenesis of rheumatic disease. Arthritis Res Ther. 10 (3): 209. 3. Volz HC,et al.(2010) The role of HMGB1/RAGE in inflammatory cardiomyopathy. Semin Thromb Hemost. 36(2): 185-94. 4. Sims GP,et al.(2010) HMGB1 and RAGE in inflammation and cancer. Annu Rev Immunol. 28: 367-88. 5. Andersson U,et al.(2010) The role of HMGB1 in the pathogenesis of rheumatic disease. Biochim Biophys Acta. 1799 (1-2): 141-8.

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Keywords

HMGB1 Protein, Human, Recombinant (hFc) HMG1 SBP 1 SBP-1 high mobility group box 1 SBP1 HMG3 HMG 3 HMG-3 HMG-1 HMG 1 recombinant recombinant-proteins proteins protein

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