CD70 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 45.7 kDa and the accession number is A0A0U5JA32.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,940 | 5日内发货 | ||
100 μg | ¥ 6,800 | 5日内发货 |
生物活性 | Immobilized Human CD27 (His & hFc Tag) at 2 μg/mL (100 μL/well) can bind Human CD70 (ECD, hFc & AVI Tag), Biotinylated, the EC50 is 8-68ng/mL. |
产品描述 | CD70 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 45.7 kDa and the accession number is A0A0U5JA32. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | hFc, Avi |
蛋白编号 | A0A0U5JA32 |
别名 | CD70 molecule, CD27LG, TNFSF7, CD27L |
蛋白构建 | The Human CD70 (NP_001243.1) (Gln39-Pro193) was expressed with a N-terminal Fc region of Human IgG1 tagged AVI tag at the N-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
蛋白纯度 | > 80 % as determined by SDS-PAGE. |
分子量 | 45.7 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | CD70, a member of the tumor necrosis factor superfamily, is restricted to activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor, CD27, is important in priming, effector functions, differentiation and memory formation of T-cells as well as plasma and memory B-cell generation. Tight control of CD70 expression is required to prevent lethal immunodeficiency. By selective transcription, CD70 is largely confined to activated lymphocytes and dendritic cells (DC). As a type II transmembrane receptor, CD70 is normally expressed on a subset of B, T and NK cells, where it plays a costimulatory role in immune cell activation. Immunohistochemical analysis of CD70 expression in multiple carcinoma types. The restricted expression pattern of CD70 in normal tissues and its widespread expression in various malignancies makes it an attractive target for antibody-based therapeutics. Investigations to exploit CD70 as a cancer target have lead to the identification of potential antibody-based clinical candidates.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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CD70 Protein, Human, Recombinant (hFc & Avi), Biotinylated CD70 molecule CD27LG TNFSF 7 TNFSF-7 TNFSF7 CD27L recombinant recombinant-proteins proteins protein