Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SHP2 protein degrader-1 is a highly efficient allosteric inhibitor targeting SHP2. It effectively induces degradation of SHP2 protein, resulting in cell apoptosis. This compound shows promising potential for studying diseases associated with SHP2.
产品描述 | SHP2 protein degrader-1 is a highly efficient allosteric inhibitor targeting SHP2. It effectively induces degradation of SHP2 protein, resulting in cell apoptosis. This compound shows promising potential for studying diseases associated with SHP2. |
体外活性 | SHP2 protein degrader-1 (compound SP4) (0-200 μM; 24 hours) inhibits the growth of Hela cells, with IC 50 of 5.77 and 4.30 nM, respectively, which are about 100 times higher than the activity of SHP099 (SHP2 inhibitor)[1]. SHP2 protein degrader-1 (compound SP4) (0-200 μM; 96 hours) induces 54.01% apoptotic death compared with 0.13% of the control at 100 nM[1]. SHP2 protein degrader-1 (compound SP4) (0-200 μM; 96 hours) induces cell cycle arrested at the G1 phase in Hela cells[1]. SHP2 protein degrader-1 (compound SP4) (0-200 μM; 96 hours) significantly inhibits the phosphorylation of JNK, Erk and p38[1]. SHP2 protein degrader-1 (compound SP4) (0-200 μM; 96 hours) inhibits RAS/MAPK signaling and cellular responses by a mechanism involving inhibition of SHP2 catalytic activity[1]. Cell Viability Assay[1]Cell Line: Hela cells Concentration: 0-200 ?μM Incubation Time: 24 hours Result: Strongly inhibited the growth of Hela cells, with IC 50 of 5.77 and 4.30 nM, respectively, which were about 100 times higher than the activity of SHP099 (SHP2 inhibitor). Apoptosis Analysis[1]Cell Line: Hela cells Concentration: 0-100 ?μM Incubation Time: 96 hours Result: Induced 54.01% apoptotic death compared with 0.13% of the control at 100 nM. While SHP099 at 1000 nM could induce 33.74% apoptotic death compared with 0.13% of the control. Cell Cycle Analysis[1]Cell Line: Hela cells Concentration: 0-100 ?μM Incubation Time: 96 hours Result: Induced cell cycle arrested at the G1 phase in Hela cells. Western Blot Analysis[1]Cell Line: Hela cells Concentration: 0-100 ?μM Incubation Time: 96 hours Result: Significantly inhibited the phosphorylation of JNK, Erk and p38. |
别名 | SHP2 protein degrader-1 |
分子量 | 908.84 |
分子式 | C42H51Cl2N11O8 |
CAS No. | 2624181-69-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SHP2 protein degrader-1 2624181-69-5 SHP-2 protein degrader-1 SHP2 protein degrader1 SHP2 protein degrader 1 Inhibitor inhibitor inhibit