mixed isomers

5(6)-CR110 [5-(and 6)-Carboxyrhodamine 110] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

5(6)-CR110, SE [5-(and 6)-Carboxyrhodamine 110, succinimidyl ester] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

5(6)-TAMRA (Mixed isomers) (5-(and-6)-Carboxytetramethylrhodamine)是一种罗丹明类荧光染料，Ex/Em = 545/580 nm，常用于DNA分析中寡核苷酸的标记。

ROX dyes have a strong red fluorescence.Their excitation and emission wavelengths are longer than those of traditional rhodamine dyes.They are used as labels for peptides, proteins, and other biological ligands.5-ROX, 6-ROx, and their mixture of 5(6) -RoX

5(6)-TRITC [Tetramethylrhodamine-5-(and-6) -Isothiocyanate] *Mixed isomers* is used to label antibodies with strong fluorescent dyes

Rhodamine B isothiocyanate (mixed isomers)是一种橙红色的罗丹明B荧光染料，具有细胞渗透性，能够与蛋白质和抗体上的胺基偶联，Ex/Em=570/595 nm，可用于荧光显微镜和流式细胞术。

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) 是一种胺反应性 pH 敏感的绿色荧光标记物，能够标记蛋白质、多肽和氨基酸，可用于体内实验检测肿瘤位置。

5(6)-Aminofluorescein (5(6)-AFM) 是一种合成 5(6)-FITC 的前体。其中5(6)-FITC 是胺活性衍生物的荧光染料。

5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) 是绿色荧光染料，普遍用于标记蛋白质。

5(6)-ROX, SE is an amine-reactive fluorescent probe that is widely used for oligonucleotide labeling and automated DNA sequencing applications.

Difluorocarboxyfluorescein NHS Ester, 6-isomer是荧光素的氟化类似物，可以与胺反应。它提供了更高的光稳定性和更低的pKa值（约4.7，相较于荧光素的6.4），在生理pH范围内其荧光对pH变化几乎不敏感。虽然二氟羧基荧光素NHS酯的混合异构体是标记蛋白质、肽和核苷酸的首选荧光染料，但在HPLC纯化中，5(6)异构体标记的肽和核苷酸信号可能会显著增宽，这会导致纯化的麻烦。用单一异构体标记的肽和核苷酸在HPLC纯化中通常具有更好的分辨率，这在结合过程中常常是必要的。